| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 251 | Gracilibacillus timonensis | TCGAGCGCACGTTGGAAT | CATGTCGTGCCCATCTGGA | 60.05 | 59.78 | 290 | 81 |
100.00%
|
100.00%
|
| Primer 252 | Gracilibacillus timonensis | TCGAGCGCACGTTGGAAT | TCCATGTCGTGCCCATCTG | 60.05 | 59.78 | 292 | 81 |
100.00%
|
100.00%
|
| Primer 253 | Gracilibacillus timonensis | TCGAGCGCACGTTGGAAT | ACTCCATGTCGTGCCCATC | 60.05 | 59.78 | 294 | 81 |
100.00%
|
100.00%
|
| Primer 254 | Gracilibacillus timonensis | TCGAGCGCACGTTGGAAT | GCTGCTGTGGAAGGTACCA | 60.05 | 59.63 | 151 | 81 |
100.00%
|
100.00%
|
| Primer 255 | Gracilibacillus timonensis | TCGAGCGCACGTTGGAAT | ACTCCATGTCGTGCCCAT | 60.05 | 58.60 | 294 | 81 |
100.00%
|
100.00%
|
| Primer 256 | Gracilibacillus timonensis | TCGAGCGCACGTTGGAAT | TGTCGTGCCCATCTGGATC | 60.05 | 59.48 | 288 | 81 |
100.00%
|
100.00%
|
| Primer 257 | Gracilibacillus timonensis | TCGAGCGCACGTTGGAAT | AACTCCATGTCGTGCCCAT | 60.05 | 59.31 | 295 | 81 |
100.00%
|
100.00%
|
| Primer 258 | Gracilibacillus timonensis | TGCAAGGTCAACGCTGGT | CCCGTACGAGTTTCCAACCA | 59.81 | 59.97 | 286 | 81 |
100.00%
|
100.00%
|
| Primer 259 | Gracilibacillus timonensis | TCCAGTAGAATTCGCAGCCAT | TCCGCTCGCAAAATGGGA | 59.51 | 59.65 | 153 | 81 |
100.00%
|
100.00%
|
| Primer 260 | Gracilibacillus timonensis | TGTGCGTTTGGTTGGGGT | TTGGCAGCCCATTGGACT | 60.04 | 59.15 | 187 | 81 |
100.00%
|
100.00%
|